Cspb promoter
WebNov 1, 1992 · The DNA sequence of the cspB promoter region is shown at the bottom. Interruption of the chromosomal copy of the cspB gene by a double-crossover … WebJul 5, 2000 · Csp proteins (CspE, CspA, CspB, and CspC) were used for in vitro transcription assays, as described in A: lane 1, no Csp protein; 1.4 μM Csp for lanes 2, 5, …
Cspb promoter
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WebApr 26, 2011 · The promoter of the cspB gene coding for PS2 was found to direct expression of the Mycobacterium tuberculosis gene coding for the fibronectin-binding protein 85A (Salim et al. 1997), the Streptoverticillium … WebJan 1, 1999 · In contrast to cspB and cspC, whose promoters and 5 UTRs share a high degree of sequence identity, cspD lacks a long 5 UTR and has a dissimilar putative promoter sequence (Graumann et al. 1997). The presence or absence of the long 5 UTR may explain the differential stationaryphase induction of B. subtilis CSPs.
WebDec 31, 2002 · AmyA expression under the control of cspB promoter was assumed to be induced when raw starch was used as the sole carbon source. These results indicate that efficient simultaneous saccharification and fermentation of raw corn starch to L-lysine were achieved by C. glutamicum secreting AmyA using the cspB promoter and signal … WebJul 5, 2000 · In E. coli, CspA, CspB, CspG, and CspI are cold-shock inducible; CspC and CspE are constitutively produced at 37°C; CspD is induced at the early stationary phase ... (Left) or T metYt1 (Right) fused to the T7 A1 promoter. (C) Effects of CspA, CspB, CspC, and CspE on transcription termination at tR2. Csp proteins (CspE, CspA, ...
WebSep 22, 2007 · Corynebacterium glutamicum is an important microorganism in the industrial production of amino acids. We engineered a strain of C. glutamicum that secretes α … WebCold shock protein CspB. Gene. cspB. Status. UniProtKB reviewed (Swiss-Prot) Organism. Bacillus subtilis (strain 168) Amino acids. 67. Protein existence ... Can act as …
WebOct 26, 2024 · The use of constitutive promoters is advantageous because they do not require expensive reagents for induction or optimized induction conditions (Yim et al., 2013). Constitutive promoters derived from the genome of C. glutamicum, such as P sodA, P gapA, P eftu, and P cspB, are known to have high expression levels
WebThe mission of the SPCB is to provide independent, verifiable evidence of proficiency in specialty pharmacy practice through a nationally recognized, accredited, and rigorous … fix that plumbing \\u0026 gasWebApr 27, 2011 · Therefore, vector pCRD314 containing tac promoter, SD sequence of tpi, and gfp gene was constructed. Second, tac promoter was replaced with promoters of PS1-encoding cspA or PS2-encoding cspB of C. glutamicum, as enhanced heterologous protein secretion was previously achieved using cspB promoter and cspA signal sequences … canning chemist nottinghamWebDec 1, 2011 · DNA fragments containing the promoter sequence of CspB from C. glutamicum and the signal sequence of CspA from C. ammoniagenes were fused to the CspB50TEV-Teriparatide gene. This synthesized construct was then inserted into the pPK4 plasmid and incorporated into the YDK010 bacterial strain, which is a derivative of wild … canning celery in pressure cookerWebunder the control of the cspB promoter were constructed and introduced into the C. glutamicum Cm strain. Immu-nostaining microscopy and flow cytometric analysis revealed that PorB-AmyA, PorC-AmyA, and PorH-AmyA were displayed on the C. glutamicum cell surface. AmyA activity was only detected in the cell fraction of C. canning cerealWebA new class of cold shock-induced proteins that may be involved in an adaptive process required for cell viability at low temperatures or may function as antifreeze proteins in … canning ceoWebApr 1, 2006 · Furthermore, the promoter of the cspB gene was mapped by 5′ rapid amplification of cDNA ends PCR and the corresponding DNA fragment was used in DNA affinity purification assays. A 30 kDa protein specifically binding to the promoter region of the cspB gene was purified. Matrix-assisted laser desorption ionization time-of-flight … canning chemist radfordWebFeb 6, 2012 · The latter is flanked by two loxP sites and constitutes of a transcriptional promoter (P-35S), the nptII coding sequence (CS-nptII), and a polyadenylation sequence from the nopaline synthase gene (T-nos). MON 87460 contains one copy of the insert at a single insertion site hosting both cspB and nptII intact expression cassettes. fix that saying