WebFeb 8, 2024 · Bacteria were identified in eggs, the female reproductive tract and the gut of adult and L3 larvae using the universal FISH Eub338 probe, which targets most bacteria, and were also seen by light and transmission electron microscopy. Those in the reproductive tract were of two different morphotypes and their sequences matched to unrelated type ... WebAug 31, 2024 · Ribosomal RNA (rRNA) fluorescence in situ hybridization (FISH) was executed using the EUB338 16S rRNA gene probe labeled with the fluorophore 5’-Cy3 …
The bacterial community associated with the sheep ... - PLOS
WebJun 19, 2024 · Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes is a key method for the detection of (uncultured) microorganisms in environmental and medical samples. A major limitation of standard FISH protocols, however, is the small number of phylogenetically distinct target organisms that can be detected … neff b6cch7an0
Water Free Full-Text A Keystone Methylobacterium Strain in …
WebJun 8, 2009 · FISH was done using an eubacterial probe EUB338 (5′-GCTGCCTCCCGTAGGAGT-3′) and a nonsense probe NSEUB338 (5′-ACTCCTACGGGAGGCAGC-3′) labeled with 6′-carboxyfluorescein Cy3 at the 5′ end ... WebMay 4, 2024 · EUB338 fluorescence in situ hybridization (FISH) was carried out following the reported methods (Huang et al. 2024). The colon tissues were fixed with 4% paraformaldehyde and prepared into 5 μm sections. ... D The occurrence of BT was assessed using EUB338 FISH detection. N = 10. E The mRNA levels of zonula … WebSep 30, 2008 · (D) Combined Muc2 immunostaining (green) as in A and FISH analysis using the general bacterial probe EUB338-Alexa Fluor 555 (red) of distal colon shows Muc2-positive goblet cells and an inner stratified (s) mucus layer on the epithelium. This layer is devoid of bacteria, which can only be detected in the outer mucus layer. neff b7742no