WitrynaAstrocytic [Cl −] i changes rapidly in response to awakening. To monitor dynamic changes in astrocytic [Cl −] i in awake freely moving or naturally sleeping mice, we used fibre photometry of astrocytes specifically expressing the fluorescent Cl − sensor mClY 37 under the Gfap-promoter to record the bulk signal from many astrocytes. mCIY … Witryna15 lip 2024 · These lightweight one-photon calcium imaging microscopes (miniscopes) have lower resolution and lack optical sectioning compared to 2 P M. In contrast, they can be mounted on the head of freely moving animals and thus allow the study of neuronal activity in behavioral paradigms that require freely-behaving rodents.
Brain Sciences Free Full-Text Neuromolecular Imaging Shows …
Witryna1 cze 2024 · Some studies have used single-photon Ca 2+ imaging in freely behaving animals successfully to elucidate the neuronal mechanisms underpinning specific behaviors [5, 6]. However, because of the relatively slow dynamics of calcium transients, it is difficult to relate calcium signals to the specific timing of behavior and fast network … WitrynaDuring this webinar, the Bruker team will demonstrate how to simultaneously image calcium in up to 4 brain areas of freely-behaving mice to map out neuronal circuits … china cooks air fryer supplier
Miniature two-photon microscopy for enlarged field-of-view, multi …
Witryna2 kwi 2024 · Method of the Year 2024: Imaging in freely behaving animals, Nature Methods, 2024; Azevedo FAC, et al. Equal numbers of neuronal and nonneuronal … Witrynatheir field of view and ability to image more than one focal plane. Wethereforedevelopedanactive,animal-actuatedrotatinghead-post that allows mice to engage in 2D navigation and freely turn their head. Mice remain fixed in the azimuthal plane and rotate around the center of the microscope field of view, allowing use of … WitrynaThe nVue system enables dual color imaging of two distinct cell populations in freely-behaving animals, empowering researchers with greater neural circuit insights in … china cooler bag